G protein-coupled estrogen receptor is not required for sex determination or ovary function in zebrafish

An important negative result: We find that G protein-coupled estrogen receptor (GPER) is not required for sex determination or ovary development in zebrafish. While nuclear estrogen receptors are clearly involved in sex determination and gonad development, Cammie’s preprint suggests that non-canonical estrogen signaling is dispensable for sex determination and gonad development.